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. 2007 Feb;13(2):322-4.
doi: 10.3201/eid1302.061032.

Rapid genome sequencing of RNA viruses

Tetsuya Mizutani  1 , Daiji Endoh, Michiko Okamoto, Kazuya Shirato, Hiroyuki Shimizu, Minetaro Arita, Shuetsu Fukushi, Masayuki Saijo, Kouji Sakai, Chang Kweng Lim, Mikako Ito, Reiko Nerome, Tomohiko Takasaki, Koji Ishii, Tetsuro Suzuki, Ichiro Kurane, Shigeru Morikawa, Hidekazu Nishimura
Affiliations

Rapid genome sequencing of RNA viruses

Tetsuya Mizutani et al. Emerg Infect Dis. 2007 Feb.

Abstract

We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.

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Figures

Figure 1
Figure 1
Overall scheme of the rapid determination of viral RNA sequence method. *By adding RNase H; WGA, whole genome amplification; †With specially designed primer sets as shown in Figure 2.
Figure 2
Figure 2
Primers used in rapid determination of viral RNA sequence method.
See this image and copyright information in PMC

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