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Comparative Study
. 2007 Apr;18(2):113-9.

DNA sequencing research group: 2006 general survey of DNA sequencing facilities

David Needleman  1 , Debbie Adam, Michelle Detwiler, Helaman Escobar, Jan Kieleczawa, Rashmi Pershard, Peter Schweitzer, Glenis Wiebe
Affiliations
Comparative Study

DNA sequencing research group: 2006 general survey of DNA sequencing facilities

David Needleman et al. J Biomol Tech. 2007 Apr.

Abstract

Over the past few years, technological advances in automated DNA sequencing have had a profound effect on the nature of DNA sequencing laboratories. To characterize the changes occurring within DNA sequencing facilities, the DNA Sequencing Research Group conducted three previous studies, in 1998, 2000, and 2003. A new general survey has been designed and conducted by the DSRG to capture the current status of DNA sequencing facilities in all sectors. Included were questions regarding facility administration, pricing, instrumentation, technology, protocols, and operation. The results of the survey are presented here, accompanied by comparisons to the previous surveys. These comparisons formed a basis for the discussion of trends within the facilities in response to the dynamics of a changing technology.

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Figures

FIGURE 1
FIGURE 1
Facility funding.
FIGURE 2
FIGURE 2
Annual salary ranges, in us dollars, for (A) directors/managers and (B) technicians.
FIGURE 3
FIGURE 3
Number of processed sequencing reactions within the year.
FIGURE 4
FIGURE 4
Assignment of sequence quality by the respondents.
FIGURE 5
FIGURE 5
Percentage of respondents reporting average turnaround time for unedited sequences.
FIGURE 6
FIGURE 6
Comparison of DNA sequencing platforms.
FIGURE 7
FIGURE 7
Type of sequencing platforms reported by respondents.
FIGURE 8
FIGURE 8
Primary DNA sequencing chemistry.
See this image and copyright information in PMC

References

    1. Wiebe GJ, Schweitzer PA, Needleman DS, Adam D, Detwiler M, Escobar H, et al. DSRG Presentation: 2006 General Survey of DNA Sequencing Facilities and Launch of the Web-based Trouble-Shooting Resource. J Biomol Tech. 2006;17:73. (Abstr) - PMC - PubMed
    1. Adam D, Kieleczawa J, Escobar H, Detwiler M, Pershad R, Needleman DS, et al. DNA Sequencing Research Group—2006 General Survey of DNA Sequencing Facilities. J Biomol Tech. 2006;17:28. (Abstr)
    1. Wiebe GJ, Pershad R, Escobar H, Hawes JW, Hunter T, Jackson-Machelski E, et al. DNA Sequencing Research Group (DSRG) 2003—A General Survey of Core DNA Sequencing Facilities. J Biomol Tech. 2003;14:231–237.
    1. Grills G, Adams PS, Dolejsi MK, Hardin S, McMinimy D, Morrison P, et al. Analysis of the Effects of Different DNA Sequencing Methods on Sequencing Quality, Creation of a Quality Control Resource, and Assessment of the Current State of the Art: Results from the 1998 ABRF DNA Sequence Research Committee Study. http://www.abrf.org/ResearchGroups/DNASequencing/EPosters/dsrg98/survey/....
    1. Leviten D, Grills G, Hardin S, Robertson M, Thannhauser T, VanEe J. Results from the DNA Sequencing Research Group’s Survey of Core Facilities, 2000. ABRF DSRG 2000 Web Poster. www.abrf.org/ResearchGroups/DNASequencing/EPosters/DSRGGeneral2k/index.htm.

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