doi: 10.1038/nmeth1051.
Epub 2007 May 13.
Microgram-scale protein structure determination by NMR
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- PMID: 17496898
- DOI: 10.1038/nmeth1051
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Microgram-scale protein structure determination by NMR
Nat Methods.
2007 Jun.
Abstract
Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mug (6 microl, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.
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