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. 2007 Jun;88(3):175-83.
doi: 10.1111/j.1365-2613.2006.00510.x.

Reduced protein expression of metastasis-related genes (nm23, KISS1, KAI1 and p53) in lymph node and liver metastases of gastric cancer

Yu Guan-Zhen  1 , Chen Ying, Ni Can-Rong, Wang Guo-Dong, Qian Jian-Xin, Wang Jie-Jun
Affiliations

Reduced protein expression of metastasis-related genes (nm23, KISS1, KAI1 and p53) in lymph node and liver metastases of gastric cancer

Yu Guan-Zhen et al. Int J Exp Pathol. 2007 Jun.

Abstract

Purpose: Metastasis remains an incurable common complication in patients with gastric cancer. A variety of theories have been proposed to explain the inefficiency of the metastatic process. To compare protein expression of metastasis-related genes (nm23, KISS1, KAI1 and p53) between primary tumours and metastatic tumours may be useful in illustrating these theories.

Methods: Metastasis-related tissue microarrays (including normal tissues, primary tumours, nodal metastases and liver metastases) were constructed. The protein expression of nm23, KISS1, KAI1 and p53 in lymph node and liver metastases from advanced gastric cancer specimens was mainly examined by immunohistochemical staining in relation to primary tumours.

Results: Immunohistochemical staining showed reduced protein expression of nm23, KISS1 and KAI1 in lymph node and liver metastases compared with primary tumours. Results for p53 were to the contrary.

Conclusions: Our investigations revealed a tendency of reduced protein expression of metastasis suppressor genes nm23, KISS1 and KAI1 in gastric cancer with the progress of metastasis. This means that the progression theory is an important determinant of metastatic efficiency.

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Figures

Figure 1
Figure 1
Tumour tissue microarray (TMA) slide. The TMA consisted of one normal, two primary tumour, and two to three nodal metastases cores from each invasive gastric cancer case placed at one line. For those with liver metastases, two cores were involved.
Figure 2
Figure 2
Results of nm23, KAI-1, KISS-1 and p53 protein levels in normal, tumour, lymph node and liver metastases.
Figure 3
Figure 3
Representative examples of IHC staining for KAI1, KISS1, nm23 and p53 (IHC ×200). N, normal tissue. The percentage and intensity of positive cells of KAI1, KISS1 and nm23 were gradually lower in metastases (especially in liver lesions) than that in primary tumour and normal tissues. T, primary tumour; L, nodal metastases; V, Lver metastases.
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References

    1. Adachi M, Taki T, Leki Y, Huang CL, Higashiyama M, Miyake M. Correlation of Kai1/CD82 gene expression with good prognosis in patients with non-small cell lung cancer. Cancer Res. 1996;56:1751–1755. - PubMed
    1. Arnaud-Dabernat S, Bourbon PM, Dierich A, Le Meur M, Daniel JY. Knockout mice as model system for studying nm23/NDP kinase gene functions. Application to the nm23-M1 gene. J. Bioenerg. Biomembr. 2003;35:19–30. - PubMed
    1. Berney CR, Fisher RJ, Yang J, Russell PJ, Crowe PJ. Protein markers in colorectal cancer: predictors of liver metastasis. Ann. Surg. 1999;230:179–184. - PMC - PubMed
    1. Debies MT, Welch DR. Genetic basis of human breast cancer metastasis. J. Mamm. Gliand. Biol. Neopl. 2002;6:441–451. - PubMed
    1. Dhar DK, Naora H, Kubota H, et al. Downregulation of KiSS-1 expression is responsible for tumor invasion and worse prognosis in gastric carcinoma. Int. J. Cancer. 2004;111:868–872. - PubMed

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