Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jun;195(2):207-13.
doi: 10.1016/0003-2697(91)90318-n.

Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases

V V Gurevich  1 , I D Pokrovskaya, T A Obukhova, S A Zozulya
Affiliations

Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases

V V Gurevich et al. Anal Biochem. 1991 Jun.

Abstract

A method for preparative in vitro mRNA synthesis is presented. The method makes it possible to generate several hundred RNA copies per molecule of linearized DNA template. The protocol is applicable to the synthesis of RNAs of differing lengths (from 200 to 3000 bases), and both SP6 and T7 RNA polymerases can be used. The transcription can be scaled up to at least 6-12 ml, yielding 4.5-18 mg of homogeneous biologically active mRNA.

PubMed Disclaimer

MeSH terms

Substances

LinkOut - more resources