Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Jul;45(7):2260-2.
doi: 10.1128/JCM.00848-07. Epub 2007 May 16.

Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms

Alma C van de Pol  1 , Anton M van Loon, Tom F W Wolfs, Nicolaas J G Jansen, Monique Nijhuis, Els Klein Breteler, Rob Schuurman, John W A Rossen
Affiliations

Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms

Alma C van de Pol et al. J Clin Microbiol. 2007 Jul.

Abstract

Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.

PubMed Disclaimer

References

    1. Bredius, R. G., K. E. Templeton, S. A. Scheltinga, E. C. Claas, A. C. Kroes, and J. M. Vossen. 2004. Prospective study of respiratory viral infections in pediatric hemopoietic stem cell transplantation patients. Pediatr. Infect. Dis. J. 23:518-522. - PubMed
    1. Casiano-Colon, A. E., B. B. Hulbert, T. K. Mayer, E. E. Walsh, and A. R. Falsey. 2003. Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults. J. Clin. Virol. 28:169-174. - PubMed
    1. McAdam, A. J. 2000. Discrepant analysis: how can we test a test? J. Clin. Microbiol. 38:2027-2029. - PMC - PubMed
    1. Murdoch, D. R. 2004. Molecular genetic methods in the diagnosis of lower respiratory tract infections. APMIS 112:713-727. - PubMed
    1. Templeton, K. E., S. A. Scheltinga, M. F. Beersma, A. C. Kroes, and E. C. Claas. 2004. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza A and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J. Clin. Microbiol. 42:1564-1569. - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources