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. 2007 Jul;73(14):4681-5.
doi: 10.1128/AEM.02491-06. Epub 2007 May 18.

Extended-spectrum beta-lactamase CTX-M-1 in Escherichia coli isolates from healthy poultry in France

Delphine Girlich  1 , Laurent Poirel, Alessandra Carattoli, Isabelle Kempf, Marie-Frédérique Lartigue, Alessia Bertini, Patrice Nordmann
Affiliations

Extended-spectrum beta-lactamase CTX-M-1 in Escherichia coli isolates from healthy poultry in France

Delphine Girlich et al. Appl Environ Microbiol. 2007 Jul.

Abstract

Genes encoding extended-spectrum beta-lactamase CTX-M-1 were detected in 12 Escherichia coli isolates recovered over a 7-month period from the ceca of healthy poultry in seven districts in France in 2005. Eleven of those strains were not clonally related and had a bla(CTX-M-1) gene located on transferable plasmids of different sizes and structures.

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Figures

FIG. 1.
FIG. 1.
PFGE of XbaI-digested genomic DNAs from 12 E. coli strains. Lanes: 1, E. coli isolate 3; 2, isolate 5; 3, isolate 12; 4, isolate 16; 5, isolate 21; 6, isolate 22; 7, isolate 33; 8, isolate 34; 9, isolate 36; 10, isolate 39; 11, isolate 47; 12, isolate 48; 13, E. coli K-12 used as an unrelated strain. Lane M contains molecular size markers (bacteriophage lambda DNA ladder), and the positions of the molecular size markers are shown to the left of the gel.
FIG. 2.
FIG. 2.
Plasmid DNA electrophoresis profile of CTX-M-1-producing E. coli isolates and E. coli TOP10(pCTXM-1) transformants (Tf). Lane 1, E. coli NTCC 50192 used as a molecular size marker; lane 2, isolate 3; lane 3, Tf 3; lane 4, isolate 5; lane 5, Tf 5; lane 6, isolate 12; lane 7, Tf 12; lane 8, isolate 21; lane 9, Tf 21; lane 10, isolate 22; lane 11, Tf 22; lane 12, isolate 33; lane 13, Tf 33; lane 14, isolate 34; lane 15, Tf 34; lane 16, isolate 36; lane 17, Tf 36; lane 18, isolate 39; lane 19, Tf 39; lane 20, isolate 47; lane 21, Tf 47; lane 22, isolate 48; lane 23, Tf 48.
FIG. 3.
FIG. 3.
Plasmid DNA fingerprinting of E. coli TOP10(pCTX-M-1) transformants (Tf) restricted with EcoRI. Lane M, molecular size markers (1-kb DNA ladder); lane 1, Tf 3; lane 2, Tf 5; lane 3, Tf 12; lane 4, Tf 21; lane 5, Tf 22; lane 6, Tf 33; lane 7, Tf 34, lane 8, Tf 36; lane 9, Tf 39; lane 10, Tf 47; lane 11, Tf 48.
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