doi: 10.1038/nbt1308.
Epub 2007 May 21.
Engineering synthetic signaling proteins with ultrasensitive input/output control
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- PMID: 17515908
- DOI: 10.1038/nbt1308
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Engineering synthetic signaling proteins with ultrasensitive input/output control
Nat Biotechnol.
2007 Jun.
Abstract
Many signaling proteins are built from simple, modular components, yet display highly complex signal-processing behavior. Here we explore how modular domains can be used to build an ultrasensitive switch--a nonlinear input/output function that is central to many complex biological behaviors. By systematically altering the number and affinity of modular autoinhibitory interactions, we show that we can predictably convert a simple linear signaling protein into an ultrasensitive switch.
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