Novel complex class 1 integron bearing an ISCR1 element in an Escherichia coli isolate carrying the blaCTX-M-14 gene

Abstract

This work identifies an ISCR1-related bla(CTX-M-14) gene, which has never been reported before, from a clinical isolate of Escherichia coli. The bla(CTX-M-14) gene was preceded by an ISCR1 element that was followed by a class 1 integron containing three different insert gene cassettes, i.e., dfrA12, orfF, and aadA2.

FIG. 1.

Schematic map of the complex class 1 integron carrying the bla_CTX-M-14 gene on plasmid pAJE0508. Open arrows, open reading frames; white ovals, 59-be; black oval, attI1 recombination site. The +1 transcription initiation site and the ATG start codon of the bla_CTX-M-14 gene are in boldface type. The −35 and −10 motifs of the putative promoter and the recombination crossover site are boxed.