Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Jun;4(6):487-9.
doi: 10.1038/nmeth1052. Epub 2007 May 21.

Pervasive combinatorial modification of histone H3 in human cells

Benjamin A Garcia  1 , James J Pesavento, Craig A Mizzen, Neil L Kelleher
Affiliations

Pervasive combinatorial modification of histone H3 in human cells

Benjamin A Garcia et al. Nat Methods. 2007 Jun.

Abstract

We developed a platform using hydrophilic interaction chromatography and high-resolution tandem mass spectrometry (MS) for analyses of histone H3 that allows comprehensive characterization of 'histone codes' at the molecular level. We identified over 150 differentially modified forms of histone H3.2 in asynchronously grown and butyrate-treated HeLa cells, revealing pervasive combinatorial modification previously unaccounted for by other techniques and providing a clarified estimate of the molecular diversity of histone H3 in mammals.

PubMed Disclaimer

Comment in

Publication types

LinkOut - more resources