Proteomic identification of differently expressed proteins responsible for osteoblast differentiation from human mesenchymal stem cells
- PMID: 17530189
- DOI: 10.1007/s11010-007-9497-3
Proteomic identification of differently expressed proteins responsible for osteoblast differentiation from human mesenchymal stem cells
Abstract
Human mesenchymal stem cells (hMSC) are a population of multipotent cells that can differentiate into osteoblasts, chondrocytes, adipocytes, and other cells. The exact mechanism governing the differentiation of hMSC into osteoblasts remains largely unknown. Here, we analyzed protein expression profiles of undifferentiated as well as osteogenic induced hMSC using 2-D gel electrophoresis (2-DE), mass spectrometry (MS), and peptide mass fingerprinting (PMF) to investigate the early gene expression in osteoblast differentiation. We have generated proteome maps of undifferentiated hMSC and osteogenic induced hMSC on day 3 and day 7. 2-DE revealed 102 spots with at least 2.0-fold changes in expression and 52 differently expressed proteins were successfully identified by MALDI-TOF-MS. These proteins were classified into 7 functional categories: metabolism, signal transduction, transcription, calcium-binding protein, protein degradation, protein folding and others. The expression of some identified proteins was confirmed by further RT-PCR analyses. This study clarifies the global proteome during osteoblast differentiation. Our results will play an important role in better elucidating the underlying molecular mechanism in hMSC differentiation into osteoblasts.
Similar articles
-
Comparing the protein expression profiles of human mesenchymal stem cells and human osteoblasts using gene ontologies.Stem Cells Dev. 2005 Aug;14(4):354-66. doi: 10.1089/scd.2005.14.354. Stem Cells Dev. 2005. PMID: 16137224
-
Proteomic analysis of human mesenchymal stromal cells derived from adipose tissue undergoing osteoblast differentiation.Cytotherapy. 2010 Jul;12(4):478-90. doi: 10.3109/14653240903580270. Cytotherapy. 2010. PMID: 20230220
-
Characterization of mesenchymal stem cell subpopulations from human amniotic membrane with dissimilar osteoblastic potential.Stem Cells Dev. 2013 Apr 15;22(8):1275-87. doi: 10.1089/scd.2012.0359. Epub 2013 Jan 29. Stem Cells Dev. 2013. PMID: 23211052
-
New insights into BMP-7 mediated osteoblastic differentiation of primary human mesenchymal stem cells.Bone. 2009 Jul;45(1):27-41. doi: 10.1016/j.bone.2009.03.656. Epub 2009 Mar 21. Bone. 2009. PMID: 19306956
-
A Perspective on Stem Cells as Biological Systems that Produce Differentiated Osteoblasts and Odontoblasts.Curr Stem Cell Res Ther. 2017;12(3):247-259. doi: 10.2174/1574888X11666161026145149. Curr Stem Cell Res Ther. 2017. PMID: 27784228 Review.
Cited by
-
Investigation of the Cell Surface Proteome of Human Periodontal Ligament Stem Cells.Stem Cells Int. 2016;2016:1947157. doi: 10.1155/2016/1947157. Epub 2016 Aug 4. Stem Cells Int. 2016. PMID: 27579043 Free PMC article.
-
Mitochondrial function and energy metabolism in umbilical cord blood- and bone marrow-derived mesenchymal stem cells.Stem Cells Dev. 2012 Mar 1;21(4):575-88. doi: 10.1089/scd.2011.0023. Epub 2011 Jul 20. Stem Cells Dev. 2012. PMID: 21615273 Free PMC article.
-
Proteomic profiling of human placenta-derived mesenchymal stem cells upon transforming LIM mineralization protein-1 stimulation.Cytotechnology. 2015 Mar;67(2):285-97. doi: 10.1007/s10616-013-9684-x. Epub 2014 Jan 28. Cytotechnology. 2015. PMID: 24468833 Free PMC article.
-
[Research progress in the mechanism of protein factors in regulating bone remodeling].Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2019 Jan 15;33(1):115-123. doi: 10.7507/1002-1892.201808059. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2019. PMID: 30644271 Free PMC article. Review. Chinese.
-
Human Mesenchymal Stem Cell-Treated Regulatory CD23+CD43+ B Cells Alleviate Intestinal Inflammation.Theranostics. 2019 Jun 24;9(16):4633-4647. doi: 10.7150/thno.32260. eCollection 2019. Theranostics. 2019. PMID: 31367246 Free PMC article.
References
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources