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. 1976 Jan;151(1):132-5.
doi: 10.3181/00379727-151-39159.

Purification and buoyant density of infectious bovine rhinotracheitis virus

L T Talens, Y C Zee

Purification and buoyant density of infectious bovine rhinotracheitis virus

L T Talens et al. Proc Soc Exp Biol Med. 1976 Jan.

Abstract

A purification scheme for infectious bovine rhinotracheitis virus utilizing rate-zonal centrifugation in a 10-40% potassium tartrate gradient was described. The density of IBRV in the potassium tartrate gradient was found to be 1.22 g/cm3. Electron microscopic examination of purified virus preparations revealed homogeneous populations of enveloped virions with minute projections on the envelope surface.

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