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Comparative Study
. 2007 Aug;45(8):2726-30.
doi: 10.1128/JCM.00321-07. Epub 2007 May 30.

Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach

Roger Dumke  1 , Nicol Schurwanz, Matthias Lenz, Markus Schuppler, Christian Lück, Enno Jacobs
Affiliations
Comparative Study

Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach

Roger Dumke et al. J Clin Microbiol. 2007 Aug.

Abstract

To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.

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Figures

FIG. 1.
FIG. 1.
(A) Schematic representation showing the location of the different repMp1 copies (indicated by arrows) in the genome of M. pneumoniae strain M129. (B) Partial alignment of the 14 repMp1 copies of M. pneumoniae strain M129 and the location of the primer (drawn arrow) and of the probe (dashed arrow) used for real-time PCR. Differing bases and gaps are indicated by boxes. Genome positions are derived from the published sequence (; GenBank accession no. U00089).
See this image and copyright information in PMC

References

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