doi: 10.1038/nprot.2007.156.
Isolation of renal proximal tubular brush-border membranes
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- PMID: 17545973
- DOI: 10.1038/nprot.2007.156
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Isolation of renal proximal tubular brush-border membranes
Nat Protoc.
2007.
Abstract
This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.
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