Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007;2(6):1356-9.
doi: 10.1038/nprot.2007.156.

Isolation of renal proximal tubular brush-border membranes

Jürg Biber  1 , Bruno Stieger, Gerti Stange, Heini Murer
Affiliations

Isolation of renal proximal tubular brush-border membranes

Jürg Biber et al. Nat Protoc. 2007.

Abstract

This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.

PubMed Disclaimer

Publication types

LinkOut - more resources