Rapid and non-invasive detection of plants senescence using a delayed fluorescence technique

Abstract

Senescence is a phase of leaf ontogeny marked by declining photosynthetic activity that is paralleled by a decline in chloroplast function. The photosystem II in a plant is considered to be the primary site where delayed fluorescence (DF) is produced. We report here a simple, rapid, and non-invasive technique for detecting plants senescence based on quantitative measurements of DF. In the experimental study, various senescence symptoms induced by age or hormones were examined in the Catharanthus roseus L. G. Don plants. Detecting the DF emissions from leaves with a home-made DF biosensor enables DF parameters of C. roseus to be produced in a short time. Meanwhile, evaluations of leaves senescence were made from measurements of chlorophyll content, ion leakage, and net photosynthesis rate (Pn) based on the consumption of CO2 in the tested plants. The results of our investigation demonstrate that the changes in DF intensity of green plants can truly reflect the changes in photosynthetic capacity and chlorophyll content during age-dependent and hormone-modulated senescence. Moreover, the DF intensity negatively correlates with ion leakage in both types of senescence. With proper calibration, DF may provide an important approach for monitoring senescence process in vivo and quantitatively evaluating senescence extent. Therefore, a DF technique could be potentially useful for less time-consuming and automated screening of the interesting mutants with genetic modifications that change the plant senescence progress.