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. 2007 Jun 5:6:17.
doi: 10.1186/1475-2859-6-17.

Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium

Muaaz Mutaz Al-Ajlani  1 , Muhammad Abid Sheikh, Zeeshan Ahmad, Shahida Hasnain
Affiliations

Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium

Muaaz Mutaz Al-Ajlani et al. Microb Cell Fact. .

Abstract

Background: Commercial medium (Pharmamedia) was investigated for the production of surfactin by Bacillus subtilis MZ-7. Different media (defined, semi-defined, and complex media) were compared for the production of surfactin after fixing the least influential variables in standardized fermentation conditions. Carbohydrate and nitrogen supplements were also tried to improve production in Pharmamedia.

Results: Surfactin production was confirmed using PCR along with other analytical techniques and monitored by RP-HPLC and MALDI-TOF-MS. We found that optimized and brain heart infusion media were best for production of surfactin (280 mg/L) and a relatively comparable production with Pharmamedia (220 mg/L), however, supplementing Pharmamedia with Fe+ (4.0 mM) and sucrose (2 g/L) leads to a maximum production of about (300 mg/L).

Conclusion: Cottonseed-derived medium proved to be a suitable substrate for the production of bioactive substances including surfactin, a useful compound in both medical and biotechnological fields. The medium provided not only higher product accumulations but at considerably lower cost with potential for large scale industrial applications.

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Figures

Figure 1
Figure 1
A) Surfactin cluster + Na+ obtained by MALDI-TOF-MS B) Amplification of sfp fragments by PCR, using DNA from B. subtilis 168, B. subtilis strain ATCC 21332, B. subtilis MZ-7 (lanes 1, 2, and 3, respectively).
Figure 2
Figure 2
Time course of growth of B. subtilis MZ-7 on two growth media. Each point is the result of 3 measurements. The error bars show the standard deviation of the measurements. The following parameters were monitored: pH of the growth medium (▲), CFU (■), and ability of samples to inhibit the growth of B. fusiformis (○).
Figure 3
Figure 3
Surfactin production by B. subtilis MZ-7 grown on different media. Each bar represents the average of 3 measurements, with error bars indicating the standard deviation. Media used were as described in Materials and Methods: Luria bretani (LB), Nutrient broth (NB), Brain heart infusion (BHI), Optimized (Optm), Minimal salt (MS), Cooper (CM), Landy (Ln), Pharmamedia (PM), and Pharmamedia supplemented with 2 g/L sucrose and 4.0 mM Fe+ (PM+).
Figure 4
Figure 4
Influence of different supplements on the production of inhibitory substances (Surfactin) in comparison to non supplemented media (control).
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