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. 2007 Apr;13(4):614-6.
doi: 10.3201/eid1304.061136.

Bluetongue in Belgium, 2006

Jean-François Toussaint  1 , Corinne Sailleau, Jan Mast, Philippe Houdart, Guy Czaplicki, Lien Demeestere, Frank VandenBussche, Wesley van Dessel, Nesya Goris, Emmanuel Bréard, Lotfi Bounaadja, Thiry Etienne, Stephan Zientara, Kris De Clercq
Affiliations

Bluetongue in Belgium, 2006

Jean-François Toussaint et al. Emerg Infect Dis. 2007 Apr.

Abstract

Bluetongue has emerged recently in Belgium. A bluetongue virus strain was isolated and characterized as serotype 8. Two new real-time reverse transcription-quantitative PCRs (RT-qPCRs) that amplified 2 different segments of bluetongue virus detected this exotic strain. These 2 RT-qPCRs detected infection earlier than a competitive ELISA for antibody detection.

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Figures

Figure 1
Figure 1
Negatively stained bluetongue viruslike particle that caused a cytopathic effect in BHK-21 cells. Scale bar = 50 nm.
Figure 2
Figure 2
A) Distribution of outbreaks of bluetongue (shaded areas) reported in Belgium from August 18 through September 14, 2006. Area I is where the disease was initially detected. B) Cycle threshold (Ct) values observed in different zones as a result of conducting reverse transcription–quantitative PCR_S5 on individual blood samples.
See this image and copyright information in PMC

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