Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Apr;13(4):614-6.
doi: 10.3201/eid1304.061136.

Bluetongue in Belgium, 2006

Jean-François Toussaint  1 , Corinne Sailleau, Jan Mast, Philippe Houdart, Guy Czaplicki, Lien Demeestere, Frank VandenBussche, Wesley van Dessel, Nesya Goris, Emmanuel Bréard, Lotfi Bounaadja, Thiry Etienne, Stephan Zientara, Kris De Clercq
Affiliations

Bluetongue in Belgium, 2006

Jean-François Toussaint et al. Emerg Infect Dis. 2007 Apr.

Abstract

Bluetongue has emerged recently in Belgium. A bluetongue virus strain was isolated and characterized as serotype 8. Two new real-time reverse transcription-quantitative PCRs (RT-qPCRs) that amplified 2 different segments of bluetongue virus detected this exotic strain. These 2 RT-qPCRs detected infection earlier than a competitive ELISA for antibody detection.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Negatively stained bluetongue viruslike particle that caused a cytopathic effect in BHK-21 cells. Scale bar = 50 nm.
Figure 2
Figure 2
A) Distribution of outbreaks of bluetongue (shaded areas) reported in Belgium from August 18 through September 14, 2006. Area I is where the disease was initially detected. B) Cycle threshold (Ct) values observed in different zones as a result of conducting reverse transcription–quantitative PCR_S5 on individual blood samples.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Tabachnick WJ. Culicoides and the global epidemiology of bluetongue virus infection. Vet Ital. 2004;40:145–50. - PubMed
    1. OIE Animal Health Department. Bluetongue – Netherlands, Belgium, Germany-OIE. ProMed. August 21, 2006. Accessed at http://www.promedmail.org, archive no.: 20060821.2353.
    1. Communication Directorate General. Bluetongue confirmed in France. EU Midday-express. [cited 2006 Aug 31]. Available from http://europa.eu.int/rapid, reference: MEX/06/0831
    1. Toussaint JF, Sailleau C, Breard E, Zientara S, de Clercq K. Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments. J. Virol. Methods. 2006; [Epub ahead of print]. PMID: 17196266 - PubMed
    1. Breard E, Sailleau C, Coupier H, Mure-Ravaud K, Hammoumi S, Gicquel B, et al. Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain. Vet Res. 2003;34:777–89. 10.1051/vetres:2003036 - DOI - PubMed

MeSH terms