Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Mar;13(3):520-2.
doi: 10.3201/eid1303.061198.

Novel hantavirus sequences in Shrew, Guinea

Boris Klempa, Elisabeth Fichet-Calvet, Emilie Lecompte, Brita Auste, Vladimir Aniskin, Helga Meisel, Patrick Barrière, Lamine Koivogui, Jan ter Meulen, Detlev H Krüger

Novel hantavirus sequences in Shrew, Guinea

Boris Klempa et al. Emerg Infect Dis. 2007 Mar.
No abstract available

PubMed Disclaimer

Figures

Figure
Figure
Maximum likelihood phylogenetic analysis of hantaviruses showing the phylogenetic placement of Tan826 (Tanganya virus, indicated by arrow) based on partial L segment nucleotide (A) and amino acid (B) sequences and partial S segment amino acid sequences (C); GenBank accession nos. EF050454 and EF050455, respectively. The values near the branches represent PUZZLE support values (4) calculated from 10,000 puzzling steps; only values ≥70% are shown. The scale bar indicates an evolutionary distance of 0.1 substitutions per position in the sequence. Gray ellipsoids indicate the 3 major hantavirus groups (panels A and C) or different genera of the Bunyaviridae family (panel B). We used 412 nt (137 aa) of the L segment (nucleotide position 2956–3367, amino acid position 974–1110) and 147 aa (nucleotide position 685–1111, amino acid position 217–364) of the putative nucleoprotein. Fragment positions were defined according to complete sequences of Hantaan virus strain 76-118 (GenBank accession nos. NC_005222 and NC_005218, respectively). The L segment fragment was amplified as described previously (4); for the S segment fragment, highly degenerated primers Cro2F (5′-AGYCCIGTIATGRGWGTIRTYGG-3′) and Cro2R (5′-AIGAYTGRTARAAIGAIGAYTTYTT-3′) were used. TREE-PUZZLE (www.tree-puzzle.de; [4]) was used to calculate the trees with HKY (panel A) and JTT (panels B and C) evolutionary models. Missing parameters were reconstructed from the data set. The following sequences were obtained from GenBank: X55901, AJ410617, X56492, DQ268652, M63194, AJ005637, L37901, AF291704, X14383, U12396, AF484424, AF133128, D10066, X56464, D10759, U15018 for L segment and M14626, L41916, S47716, DQ268650, M32750, Z69991, L25784, AF291702, AY526097 for S segment analysis.
See this image and copyright information in PMC

Comment on

  • Hantaviruses: a global disease problem.
    Schmaljohn C, Hjelle B. Schmaljohn C, et al. Emerg Infect Dis. 1997 Apr-Jun;3(2):95-104. doi: 10.3201/eid0302.970202. Emerg Infect Dis. 1997. PMID: 9204290 Free PMC article. Review.

References

    1. Schmaljohn CS, Hjelle B. Hantaviruses: a global disease problem. Emerg Infect Dis. 1997;3:95–104. - PMC - PubMed
    1. Kruger DH, Ulrich R, Lundkvist A. Hantavirus infections and their prevention. Microbes Infect. 2001;3:1129–44. 10.1016/S1286-4579(01)01474-5 - DOI - PubMed
    1. Mertz GJ, Hjelle BL, Bryan RT. Hantavirus infection. Adv Intern Med. 1997;42:369–421. - PubMed
    1. Schmidt HA, Strimmer K, Vingron M, von Haeseler A. TREE-PUZZLE: Maximum likelihood phylogenetic analysis using quartets and parallel computing. Bioinformatics. 2002;18:502–4. 10.1093/bioinformatics/18.3.502 - DOI - PubMed
    1. Klempa B, Fichet-Calvet E, Lecompte E, Auste B, Aniskin V, Meisel H, et al. Hantavirus in African wood mouse, Guinea. Emerg Infect Dis. 2006;12:838–40. - PMC - PubMed

Publication types

Associated data

LinkOut - more resources