[A modified method for phosphoribosyl pyrophosphate synthesis]
[Article in
Russian]
- PMID: 175573
Item in Clipboard
[A modified method for phosphoribosyl pyrophosphate synthesis]
[Article in
Russian]
Vopr Med Khim.
1975 Nov-Dec.
Abstract
A modified method for synthesis of phosphoribosyl pyrophosphate (PRPP) from ribose-5-phosphate and ATP in presence of ribose phosphate pyrophosphokinase (RPPPK) was developed. RPPPK was isolated from extracts of acetone powder of rabbit liver tissue, using alcohol fractionation of proteins. The protein fraction, containing RPPPK, was isolated at 35% saturation with 96% ethyl alcohol. The identification of the synthesized preparation of PRPP was carried out in enzymatic reaction with adenine- and inosine phosphoribosyl transferases, using labelled adenine and guanine. The obtained product of reaction (AMP and GMP) were precipitated with lantane chloride.
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