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. 1991 Dec 16;181(2):507-12.
doi: 10.1016/0006-291x(91)91217-z.

Nucleotide sequence of the Clostridium thermocellum laminarinase gene

V V Zverlov  1 , D A Laptev, V I Tishkov, G A Velikodvorskaja
Affiliations

Nucleotide sequence of the Clostridium thermocellum laminarinase gene

V V Zverlov et al. Biochem Biophys Res Commun. .

Abstract

The sequence presented (1022 bp) shows the Clostridium thermocellum laminarinase gene (lam1) and its flanking regions. The gene lam1 comprises an open reading frame of 726 nt, encoding a 242-aa protein with predicted Mr 27661. The ORF startswith the translation initiation codon ATG. This ATG codon is preceded at a spacing of 7 bp by a potential ribosome binding site (GGAGGT). A putative signal peptide was identified (the potential cleavage site is between position 27-28 aa). The comparison of the primary protein sequence with other beta-1, 3-1, 4-glucanases showed extensive homology for Bacillus amyloliqefaciens and Bacillus subtilis glucanases (identity-46.7%; similarity-57.0%).

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