doi: 10.1515/znc-1975-9-1002.
Reactions of essential sulfhydryl residues of dehydrogenases with nicotinamide-(S-methylmercury-thioinosine) dinucleotide
- PMID: 175596
- DOI: 10.1515/znc-1975-9-1002
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Reactions of essential sulfhydryl residues of dehydrogenases with nicotinamide-(S-methylmercury-thioinosine) dinucleotide
Z Naturforsch C Biosci.
1975 Sep-Oct.
Abstract
Nicotinamide-(S-methylmercury-thioinosine) dinucleotide was formed by reaction of nicotinamide-(6-thiopurine) dinucleotide with methylmercury chloride. The compound exhibits coenzyme properties in the test with LDH (Km=1.5 X 10(-4) M, Vmax=12500) and LADH (Km=1.7 X 10(-4) M, Vmax=27) and inactivates YADH and GAPDH. From incubations with LDH and LADH the mercury containing coenzyme could be regarded by column be qualified for the X-ray structure analysis of the coenzyme-enzyme complex for some dehyrogenases based on the proportion of the heavy metal.
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