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. 2007 Jun 26;581(16):3076-80.
doi: 10.1016/j.febslet.2007.05.073. Epub 2007 Jun 4.

Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation

Danièle Adamski  1 , Jean-François Mayol, Nadine Platet, François Berger, Francis Hérodin, Didier Wion
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Free article

Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation

Danièle Adamski et al. FEBS Lett. .
Free article

Abstract

Accumulative evidence demonstrates that normal as well as cancer stem cells can be identified as a side population following Hoechst 33342 staining and flow cytometric analysis. This popular method is based on the ability of stem cells to efflux this fluorescent vital dye. We demonstrate that Hoechst 33342 can affect cell differentiation, suggesting potential complications in the interpretation of data.

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