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. 2007 May 1;63(Pt 5):409-11.
doi: 10.1107/S1744309107016004. Epub 2007 Apr 14.

Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana

Mamta Bajaj  1 , Hideaki Moriyama
Affiliations

Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana

Mamta Bajaj et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 A resolution using Cu K alpha radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.90, b = 70.86 A, c = 75.55 A. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a V(M) of 1.8 A3 Da(-1).

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Figures

Figure 1
Figure 1
A crystal of dUTPase from A. thaliana.
See this image and copyright information in PMC

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