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. 2007 Jun;45(2):95-102.
doi: 10.3347/kjp.2007.45.2.95.

Changes of cytokine mRNA expression and IgG responses in rats infected with Capillaria hepatica

Dong Kwan Kim  1 , Kyoung Hwan Joo, Myung Sook Chung
Affiliations

Changes of cytokine mRNA expression and IgG responses in rats infected with Capillaria hepatica

Dong Kwan Kim et al. Korean J Parasitol. 2007 Jun.

Abstract

The mRNA expression of several cytokines was evaluated in splenocytes and mesenteric lymph node (MLN) cells of rats infected with Capillaria hepatica by reverse-transcription (RT)-PCR until week 12 after infection. IgG1 and IgG2a, which are associated with Th1 and Th2 response, respectively, were also assessed by ELISA. The results indicated that the majority of cytokines, including the Th1 (IL-2 and IFN-gamma) and Th2 cytokines (IL-4, IL-5 and IL- 10) were expressed at maximal levels during the early stage of infection (after week 1-2), and the ELISA data also evidenced a similar pattern of changes in IgG1 and IgG2a. Th1 and Th2 cytokines responded in a similar fashion in this rat model. The expression of cytokines in splenocytes was significantly higher than that in MLN cells, thereby indicating that cytokine production is controlled more by spleen than by MLN. In addition, the observation that IFN-gamma expression increased unexpectedly at the time of maximal egg production (6 weeks after infection) indicated that IFN- gamma is a cytokine reacting against egg production. However, increased IL-5 expression occurring in tandem with worm activity indicated that the activity of C. hepatica might be controlled by IL-5 expression.

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Figures

Fig. 1
Fig. 1
Ethidium bromide-stained 2% agarose gels containing RT-PCR products of cytokine expression profiles in the spleen and mesenteric lymph node (MLN) of normal rats and Capillaria hepatica-infected rats. Tissues were pooled from each group.
Fig. 2
Fig. 2
Expression of IFN-γ, IL-2, IL-4, IL-5, and IL-10 in rats infected with Capillaria hepatica. RNA was purified from the spleen and MLN for various infection times from infected and uninfected rats (control). Tissues were pooled from each group. Results from one representative experiment out of 3 kinetic studies are presented as fold increase.
Fig. 3
Fig. 3
Changes of IgG isotype of rats infected with Capillaria hepatica. a. IgG1, b. IgG2a.
See this image and copyright information in PMC

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