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. 2007 Jul;8(7):639-43.
doi: 10.1038/sj.embor.7400988. Epub 2007 Jun 15.

Translation mechanism and regulation: old players, new concepts. Meeting on translational control and non-coding RNA

Martin Holcik  1 , Tatyana V Pestova
Affiliations

Translation mechanism and regulation: old players, new concepts. Meeting on translational control and non-coding RNA

Martin Holcik et al. EMBO Rep. 2007 Jul.
No abstract available

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Figures

Figure 1
Figure 1
A simplified model of the mechanism of cap-dependent initiation. eIF2·GTP/Met-tRNAiMet ternary complex, eIFs 3, 1, 1A, 5 and a 40S subunit form a 43S pre-initiation complex, which initially binds to the 5′-proximal region of mRNA. The secondary structure of the mRNA is unwound in an ATP-dependent manner by the cooperative action of eIFs 4A, 4B, 4F and poly(A) binding protein (PABP). This complex then scans downstream to the initiation codon, where it forms a 48S initiation complex with an established codon–anticodon interaction. Joining of a 60S subunit is mediated by eIF5, which induces hydrolysis of eIF2-bound GTP and partial dissociation of eIF2-GDP from a 40S subunit, and eIF5B, which promotes subunit joining and dissociation of other factors. For simplicity, PABP bound to the 3′-poly(A) tail and recycling of eIF2·GDP by eIF2B are not shown. Met, methionine.
Figure 2
Figure 2
A simplified model of translation regulation by stress. Various stress, mitogen and cytokine signals evoke activation of distinct signalling pathways resulting in the reduction of protein synthesis. Activation of eIF2α kinases (PKR-like ER kinase (PERK); haem-regulated inhibitor kinase (HRI); double-stranded RNA-activated protein kinase (PKR); general control non-derepressible-2 (GCN2)) results in the reduction of available ternary complex, whereas activation of the mammalian target of rapamycin (mTOR) and/or stress-activated MAP kinase pathways (SAPK) impinges on the activation of messenger RNA and its recruitment into the 48S initiation complex. For simplicity, only selected factors are shown. Met, methionine; P, phosphate.
None
The meeting on Translational Control and Non-Coding RNA took place between 8 and 12 November 2006, in Nove Hrady, the Czech Republic, and was organized by M. Pospisek and L. Valasek.
See this image and copyright information in PMC

References

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    1. Hinnebusch AG, Dever TE, Asano K (2007) Mechanism of translation in the yeast Saccharomyces cerevisiae. In Translational Control in Biology and Medicine, MB Mathews, N Sonenberg, JWB Hershey (eds), pp 225–268. New York, USA: Cold Spring Harbor Laboratory Press
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