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. 2007 Jul;4(7):555-7.
doi: 10.1038/nmeth1062. Epub 2007 Jun 17.

Bright monomeric red fluorescent protein with an extended fluorescence lifetime

Ekaterina M Merzlyak  1 , Joachim Goedhart, Dmitry Shcherbo, Mariya E Bulina, Aleksandr S Shcheglov, Arkady F Fradkov, Anna Gaintzeva, Konstantin A Lukyanov, Sergey Lukyanov, Theodorus W J Gadella, Dmitriy M Chudakov
Affiliations

Bright monomeric red fluorescent protein with an extended fluorescence lifetime

Ekaterina M Merzlyak et al. Nat Methods. 2007 Jul.

Abstract

Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.

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