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. 1991 Dec;29(12):2735-40.
doi: 10.1128/jcm.29.12.2735-2740.1991.

Quantitative microculture system with standardized inocula for strain typing, susceptibility testing, and other physiologic measurements with Candida albicans and other yeasts

F C Odds  1
Affiliations

Quantitative microculture system with standardized inocula for strain typing, susceptibility testing, and other physiologic measurements with Candida albicans and other yeasts

F C Odds. J Clin Microbiol. 1991 Dec.

Abstract

A method for physiologic testing of Candida albicans and other yeasts is described in which inocula with reproducible cell concentrations are prepared from cultures in a glucose-limited medium and growth turbidities of test cultures in microplates are expressed quantitatively as a percentage of control growth. The method gave good within-plate and run-to-run reproducibilities and was applicable both to strain phenotype discrimination in C. albicans and to susceptibility tests of yeasts with azole antifungal agents.

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