Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus
- PMID: 17581936
- PMCID: PMC1951245
- DOI: 10.1128/JCM.00759-07
Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus
Abstract
Ten samples containing various amounts of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, methicillin-resistant Staphylococcus epidermidis (MRSE), and combinations thereof were distributed to 51 laboratories for molecular diagnostics testing. Samples containing 10(2) to 10(3) MRSA cells were frequently reported to be negative. MRSE samples were scored as negative by all commercial tests but by only two out of three in-house tests.
References
-
- Brown, D. F., D. I. Edwards, P. M. Hawkey, D. Morrison, G. L. Ridgway, K. J. Towner, M. W. Wren, and the Joint Working Party of the British Society for Antimicrobial Chemotherapy, Hospital Infection Society, Infection Control Nurses Association. 2005. Guidelines for the laboratory diagnosis and susceptibility testing of methicillin resistant Staphylococcus aureus (MRSA). J. Antimicrob. Chemother. 56:1000-1018. - PubMed
-
- Donoso Mantke, O., S. W. Aberle, T. Avsic-Zupanc, M. Labuda, and M. Niedrig. 2007. Quality control assessment for the PCR diagnosis of tick-borne encephalitis virus infections. J. Clin. Virol. 38:73-77. - PubMed
-
- Hodgson, J., M. Zuckerman, and M. Smith. 2007. Development of a novel internal control for a real time PCR for HSV DNA types 1 and 2. J. Clin. Virol. 38:217-220. - PubMed
-
- Noordhoek, G. T., S. Mulder, P. Wallace, and A. M. Van Loon. 2004. Multicentre quality control study for detection of Mycobacterium tuberculosis in clinical samples by nucleic acid amplification methods. Clin. Microbiol. Infect. 10:295-301. - PubMed
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