Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Aug;45(8):2723-5.
doi: 10.1128/JCM.00015-07. Epub 2007 Jun 20.

Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae

K F Anderson  1 , D R Lonsway, J K Rasheed, J Biddle, B Jensen, L K McDougal, R B Carey, A Thompson, S Stocker, B Limbago, J B Patel
Affiliations

Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae

K F Anderson et al. J Clin Microbiol. 2007 Aug.

Abstract

The Klebsiella pneumoniae carbapenem (KPC) beta-lactamase occurs in Enterobacteriaceae and can confer resistance to all beta-lactam agents including carbapenems. The enzyme may confer low-level carbapenem resistance, and the failure of susceptibility methods to identify this resistance has been reported. Automated and nonautomated methods for carbapenem susceptibility were evaluated for identification of KPC-mediated resistance. Ertapenem was a more sensitive indicator of KPC resistance than meropenem and imipenem independently of the method used. Carbapenemase production could be confirmed with the modified Hodge test.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
The modified Hodge test Mueller-Hinton agar plate was inoculated with a 1:10 dilution of a 0.5 McFarland suspension of E. coli ATCC 25922 and streaked for confluent growth using a swab. A 10-μg imipenem disk was placed in the center, and each test isolate was streaked from the disk to the edge of the plate. Isolate A is a KPC producer and positive by the modified Hodge test. Isolates B, C, D, and E do not produce a carbapenemase and are negative by the test.
See this image and copyright information in PMC

References

    1. Alba, J., Y. Ishii, K. Thomson, E. S. Moland, and K. Yamaguchi. 2005. Kinetics study of KPC-3, a plasmid-encoded class A carbapenem-hydrolyzing β-lactamase. Antimicrob. Agents Chemother. 49:4760-4762. - PMC - PubMed
    1. Bradford, P. A., S. Bratu, C. Urban, M. Visalli, N. Mariano, D. Landman, J. J. Rahal, S. Brooks, S. Cebular, and J. Quale. 2004. Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 β-lactamases in New York City. Clin. Infect. Dis. 39:55-60. - PubMed
    1. Bradford, P. A., C. Urban, N. Mariano, S. J. Projan, J. J. Rahal, and K. Bush. 1997. Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC β-lactamase, and the loss of an outer membrane protein. Antimicrob. Agents Chemother. 41:563-569. - PMC - PubMed
    1. Bratu, S., D. Landman, M. Alam, E. Tolentino, and J. Quale. 2005. Detection of KPC carbapenem-hydrolyzing enzymes in Enterobacter spp. from Brooklyn, New York. Antimicrob. Agents Chemother. 49:776-778. - PMC - PubMed
    1. Bratu, S., M. Mooty, S. Nichani, D. Landman, C. Gullans, B. Pettinato, U. Karumudi, P. Tolaney, and J. Quale. 2005. Emergence of KPC-possessing Klebsiella pneumoniae in Brooklyn, New York: epidemiology and recommendations for detection. Antimicrob. Agents Chemother. 49:3018-3020. - PMC - PubMed

Publication types

MeSH terms