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. 2007 Dec;143(2):247-52.
doi: 10.1016/j.jss.2007.02.042. Epub 2007 Jun 20.

Depletion of glutathione by buthionine sulfoximine decreases random-pattern skin flap viability in rats

Yusuf Kenan Coban  1 , Yusuf Ergun, Harun Ciralik
Affiliations

Depletion of glutathione by buthionine sulfoximine decreases random-pattern skin flap viability in rats

Yusuf Kenan Coban et al. J Surg Res. 2007 Dec.

Abstract

Background: Glutathione (GSH) is one of the most highly concentrated intracellular antioxidants. Exogenous GSH has been shown to increase random-pattern skin flap survival. However, the effects of endogenous GSH depletion on random-pattern skin flap viability have never been studied.

Materials and methods: To evaluate the effects of systemic glutathione depletion on random-pattern skin flap survival in rats, 28 Wistar albino rats were divided into control, sham, and BSO (buthionine sulfoximide, a selective inhibitor for gamma-glutamylcysteine synthetase) groups. Dorsal, cranial-based random-pattern skin-flaps were elevated and the percentage of flap necrosis was measured in all rats at the postoperative day 7.

Results: BSO-treated rats showed increased skin flap necrosis when compared with untreated animals (P < 0.001). High-dose BSO treatment group had more clinically evident necrosis than low dose group (P < 0.05).

Conclusions: This study reveals the importance of endogenous GSH for random skin-flap viability.

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