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. 2007;46(29):5528-31.
doi: 10.1002/anie.200701491.

Squaraine rotaxanes: superior substitutes for Cy-5 in molecular probes for near-infrared fluorescence cell imaging

James R Johnson  1 , Na Fu, Easwaran Arunkumar, W Matthew Leevy, Seth T Gammon, David Piwnica-Worms, Bradley D Smith
Affiliations

Squaraine rotaxanes: superior substitutes for Cy-5 in molecular probes for near-infrared fluorescence cell imaging

James R Johnson et al. Angew Chem Int Ed Engl. 2007.
No abstract available

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Figures

Figure 1
Figure 1
Fluorescence microscopy images of live mammalian cells treated with 1. Panel A: Endoplasmic reticulum staining of a single CHO cell. Panel B: Lipid droplet staining in A549 cells. Panel C: Multicolor image of a single COS-7 cell treated with H33342 (blue), FM1-43 (green), and 1 (red). Panel D: MCF7 cells 144 hours after a single treatment with 1. Single color images were left in grayscale to preserve image detail and contrast. Each scale bar corresponds to 20 μM.
Figure 2
Figure 2
Montage from a larger movie showing binary fission of E. coli cells stained with 3. The cells were imaged, using fluorescence microscopy, every 5 seconds for 30 minutes. The times stated in each panel correspond to the movie time point and the scale bar represents 2 μM.
Figure 3
Figure 3
Optical image of a live mouse with subcutaneous injections of S. aureus and E. coli that were pre-labeled with 3. The entire animal was irradiated with filtered light of wavelength 625±40 nm and an image of emission intensity at 670±20 nm was collected by a CCD camera during a 5 s acquisition period.
See this image and copyright information in PMC

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