Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Sep;24(9):425-8.
doi: 10.1007/s10815-007-9145-6. Epub 2007 Jun 27.

Sperm motility stimulation and preservation with various concentrations of follicular fluid

Chatpavit Getpook  1 , Suwadee Wirotkarun
Affiliations

Sperm motility stimulation and preservation with various concentrations of follicular fluid

Chatpavit Getpook et al. J Assist Reprod Genet. 2007 Sep.

Abstract

Purpose: The purpose of this study is to evaluate the optimal concentrations of follicular fluid (FF) for sperm motility stimulation and preservation.

Methods: Thirty normal semen samples and FF from women in an in vitro fertilization programme were used in an experiment. The semen was processed and incubated in Earle culture media with 20, 50, 80, and 100% FF (v/v) with controls. Sperm motility was evaluated and followed up for 48 h.

Results: FF could stimulate progressive sperm motility at all concentrations and last for at least 12 h. However, at more than 50% v/v of FF, sperm demonstrated a rapid decline in progressive motility after 12 h of incubation compared to other concentrations and the control group.

Conclusion: FF can stimulate the sperm motility properly at not more than 50% (v/v) concentration.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Zhu J, Pacey AA, Barratt CLR, Cooks ID, Lippes J. The sequential effects of human cervical mucus, oviductal fluid, and follicular fluid on sperm function. Fertil Steril. 1994;61:1129–1135. - PubMed
    1. Hong CY, Chao HT, Lee SL, Wei YH. Modification of human sperm function by human follicular fluid: a review. Int J Androl. 1993;16:92–96. doi: 10.1111/j.1365-2605.1993.tb01160.x. - DOI - PubMed
    1. Ralt D, Manor M, Cohen-Dayag A, Tur-Kaspa I, Ben-Shlomo I, Makler A, et al. Chemotaxis and chemokinesis of human spermatozoa to follicular factor. Biol Reprod. 1994;50:774–785. doi: 10.1095/biolreprod50.4.774. - DOI - PubMed
    1. Mendoza C, Tesarik J. Effect of follicular fluid on sperm movement characteristics. Fertil Steril. 1990;50:1135–1139. - PubMed
    1. Tesarik J. Comparison of acrosome reaction-inducing activities of human cumulus oophorus, follicular fluid and ionophore A23187 in human sperm population of proven fertilizing ability in vitro. J Reprod Fertil. 1985;74:383–388. doi: 10.1530/jrf.0.0740383. - DOI - PubMed

Publication types

Substances

LinkOut - more resources