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Comparative Study
. 2007 Sep;45(9):3072-6.
doi: 10.1128/JCM.01131-07. Epub 2007 Jun 27.

Use of quantitative PCR and culture methods to characterize ecological flux in bacterial biofilms

F Dalwai  1 , D A Spratt, J Pratten
Affiliations
Comparative Study

Use of quantitative PCR and culture methods to characterize ecological flux in bacterial biofilms

F Dalwai et al. J Clin Microbiol. 2007 Sep.

Abstract

An in vitro model of supragingival plaque associated with gingivitis was characterized by traditional culture techniques, comparative 16S rRNA gene sequencing of isolates, and quantitative PCR (QPCR). Actinomyces naeslundii, Prevotella spp., and Porphyromonas gingivalis increased under conditions emulating gingivitis. Gram-negative species and total bacteria were dramatically underestimated by culture compared to the estimates obtained by QPCR.

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Figures

FIG. 1.
FIG. 1.
Different species/genera represented as a percentage of the total number of bacteria by culture and QPCR. Solid bars, percentages in biofilms grown under conditions emulating health; shaded bars, percentages in biofilms grown under conditions emulating gingivitis.
See this image and copyright information in PMC

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