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. 2007 Nov 15;370(2):255-7.
doi: 10.1016/j.ab.2007.06.002. Epub 2007 Jun 8.

A novel HPLC method reveals that precipitation of 2'-deoxyadenosine 5'-monophosphate with lithium perchlorate/acetone leads to base depurination

David L Evers  1 , Carol B Fowler, Robert E Cunningham, Jeffrey T Mason, Timothy J O'Leary
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A novel HPLC method reveals that precipitation of 2'-deoxyadenosine 5'-monophosphate with lithium perchlorate/acetone leads to base depurination

David L Evers et al. Anal Biochem. .
No abstract available

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HPLC analysis of adenine-containing species. AMP represents adenosine 5′-monophosphate, Ado is adenosine, Ade abbreviates adenine base, the d- prefix indicates 2′-deoxy analogs, and MeCN is an acronym for acetonitrile. The top chromatogram shows standards, while the bottom two chromatograms show dAMP standards precipitated by different methods. The circled peak of the middle chromatogram was identified as Ade.
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