Antimutagenic potential of curcumin on chromosomal aberrations in Allium cepa

Abstract

Turmeric has long been used as a spice and food colouring agent in Asia. In the present investigation, the antimutagenic potential of curcumin was evaluated in Allium cepa root meristem cells. So far there is no report on the biological properties of curcumin in plant test systems. The root tip cells were treated with sodium azide at 200 and 300 microg/ml for 3 h and curcumin was given at 5, 10 and 20 microg/ml for 16 h, prior to sodium azide treatment. The tips were squashed after colchicine treatment and the cells were analyzed for chromosome aberration and mitotic index. Curcumin induces chromosomal aberration in Allium cepa root tip cells in an insignificant manner, when compared with untreated control. Sodium azide alone induces chromosomal aberrations significantly with increasing concentrations. The total number of aberrations was significantly reduced in root tip cells pretreated with curcumin. The study reveals that curcumin has antimutagenic potential against sodium azide induced chromosomal aberrations in Allium cepa root meristem cells. In addition, it showed mild cytotoxicity by reducing the percentage of mitotic index in all curcumin treated groups, but the mechanism of action remains unknown. The antimutagenic potential of curcumin is effective at 5 microg/ml in Allium cepa root meristem cells.

Fig. 1

Chemical structures of curcumin and sodium azide. (a) Curcumin; (b) Sodium azide

Fig. 1

Chemical structures of curcumin and sodium azide. (a) Curcumin; (b) Sodium azide

Fig. 2

Photographs of smear preparations of root tips of Allium cepa showing different kinds of chromosomal aberrations. (a) Chromosome break and gap at 200 µg/ml of NaN₃; (b) Single chromatid breaks at 20 µg/ml of curcumin and 200 µg/ml of NaN₃; (c) Single chromatid exchange at 20 µg/ml of curcumin; (d) Fragments at 300 µg/ml of NaN₃; (e) Normal metaphase at 5 µg/ml of curcumin

Fig. 2

Photographs of smear preparations of root tips of Allium cepa showing different kinds of chromosomal aberrations. (a) Chromosome break and gap at 200 µg/ml of NaN₃; (b) Single chromatid breaks at 20 µg/ml of curcumin and 200 µg/ml of NaN₃; (c) Single chromatid exchange at 20 µg/ml of curcumin; (d) Fragments at 300 µg/ml of NaN₃; (e) Normal metaphase at 5 µg/ml of curcumin

Fig. 2

Photographs of smear preparations of root tips of Allium cepa showing different kinds of chromosomal aberrations. (a) Chromosome break and gap at 200 µg/ml of NaN₃; (b) Single chromatid breaks at 20 µg/ml of curcumin and 200 µg/ml of NaN₃; (c) Single chromatid exchange at 20 µg/ml of curcumin; (d) Fragments at 300 µg/ml of NaN₃; (e) Normal metaphase at 5 µg/ml of curcumin

Fig. 2

Photographs of smear preparations of root tips of Allium cepa showing different kinds of chromosomal aberrations. (a) Chromosome break and gap at 200 µg/ml of NaN₃; (b) Single chromatid breaks at 20 µg/ml of curcumin and 200 µg/ml of NaN₃; (c) Single chromatid exchange at 20 µg/ml of curcumin; (d) Fragments at 300 µg/ml of NaN₃; (e) Normal metaphase at 5 µg/ml of curcumin

Fig. 2

Photographs of smear preparations of root tips of Allium cepa showing different kinds of chromosomal aberrations. (a) Chromosome break and gap at 200 µg/ml of NaN₃; (b) Single chromatid breaks at 20 µg/ml of curcumin and 200 µg/ml of NaN₃; (c) Single chromatid exchange at 20 µg/ml of curcumin; (d) Fragments at 300 µg/ml of NaN₃; (e) Normal metaphase at 5 µg/ml of curcumin