[Voltage-dependent anion channels (VDAC) on human sperm membrane]
- PMID: 17615971
[Voltage-dependent anion channels (VDAC) on human sperm membrane]
Abstract
Objective: To further study gene expression and characterization of voltage-dependent anion channels (VDACs) on human spermatozoa.
Methods: VDACs were cloned by PCR from the testis cDNA library. Recombinant human sperm VDACs were produced in E. coli system by molecular cloning technology. Sperm membrane protein was extracted by 1% Triton X-100 and separated by chloroform/methanol.
Results: The gene expression of VDACs was found in the human testis cDNA library and VDAC protein was detected located on the sperm membrane by alpha-helix.
Conclusion: VDAC proteins, abundant on the human sperm membrane and responsible for anion transportation, play an important role in sperm signaling transduction and fertility.
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