doi: 10.1128/JB.00491-07.
Epub 2007 Jul 6.
Artificial control of nitrate respiration through the lac promoter permits the assessment of oxygen-mediated posttranslational regulation of the nar operon in Pseudomonas aeruginosa
Affiliations
- PMID: 17616601
- PMCID: PMC1951927
- DOI: 10.1128/JB.00491-07
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Artificial control of nitrate respiration through the lac promoter permits the assessment of oxygen-mediated posttranslational regulation of the nar operon in Pseudomonas aeruginosa
J Bacteriol.
2007 Sep.
Abstract
In this study, oxygen and nitrate regulation of transcription and subsequent protein expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to assess both transcriptional and posttranslational regulation by oxygen during physiological whole-cell nitrate reduction.
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References
-
- Arai, H., Y. Igarashi, and T. Kodama. 1995. Expression of the nir and nor genes for denitrification of Pseudomonas aeruginosa requires a novel Crp/Fnr-related transcriptional regulator, Dnr, in addition to Anr. FEBS Lett. 371:73-76. - PubMed
-
- Arai, H., T. Kodama, and Y. Igarashi. 1997. Cascade regulation of the two CBP/FNR-related transcriptional regulators (ANR and DNR) and the denitrification enzymes in Pseudomonas aeruginosa. Mol. Microbiol. 25:1141-1148. - PubMed
-
- Chiang, R. C., R. Cavicchioli, and R. P. Gunsalus. 1992. Identification and characterization of narQ, a second nitrate sensor for nitrate-dependent gene regulation in Escherichia coli. Mol. Microbiol. 6:1919-1923. - PubMed
-
- Denis, K. S., F. M. Dias, and J. J. Rowe. 1990. Oxygen regulation of nitrate transport by diversion of electron flow in Escherichia coli. J. Biol. Chem. 265:18095-18097. - PubMed
-
- Dias, F. M., R. M. Ventullo, and J. J. Rowe. 1990. Regulation and energization of nitrate transport in a halophilic Pseudomonas stutzeri. Biochem. Biophys. Res. Commun. 166:424-430. - PubMed
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