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Comparative Study
. 1976 Feb;3(2):149-56.
doi: 10.1128/jcm.3.2.149-156.1976.

Comparison of a microneutralization test in cell culture and virus neutralization test in embryonated eggs for determining infectious bronchitis virus antibodies

R E Wooley, J Brown, R B Davis, J L Blue, P D Lukert
Comparative Study

Comparison of a microneutralization test in cell culture and virus neutralization test in embryonated eggs for determining infectious bronchitis virus antibodies

R E Wooley et al. J Clin Microbiol. 1976 Feb.

Abstract

A microneutralization test (MNT) system utilizing cytopathic effect end points was effective in determing neutralization indexes for infectious bronchitis virus antibodies. The system is reproducible within 1 index unit at the 95% level of probability. Comparison of the MNT to tests in eggs resulted in a positive correlation (B =0.81), which was significant (P greater than 0.01). The quantitative dose-response relationship of the MNT is linear (P greater than 0.005), with the 95% prediction limits fitting between one 10-fold dilution.

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