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. 2007 Jun;102(3):206-11.
doi: 10.1016/j.actatropica.2007.05.002. Epub 2007 May 22.

Evaluation of the EIE-IgM-Leptospirose assay for the serodiagnosis of leptospirosis

Alan J A McBride  1 , Fernanda A Pereira, Emilson D da Silva, Rosan B de Matos, Edimilson D da Silva, Antônio G P Ferreira, Mitermayer G Reis, Albert I Ko
Affiliations

Evaluation of the EIE-IgM-Leptospirose assay for the serodiagnosis of leptospirosis

Alan J A McBride et al. Acta Trop. 2007 Jun.

Abstract

Access to low-cost, effective diagnosis for leptospirosis is urgently needed in developing countries. The EIE-IgM-Leptospirose, a kit produced for public health laboratories in Brazil, was shown to have a sensitivity of 76% (77 of 102 patients) and 100% (102 of 102 patients) during acute and convalescent-phase leptospirosis, respectively, and a specificity of 93-100% (total healthy and patient control subjects evaluated, 486). These findings indicate that the assay will be useful for diagnosis of this emerging infectious disease in Brazil and other developing countries.

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Figures

Fig. 1
Fig. 1
Persistence of anti-whole Leptospira IgM antibodies detected by the EIE-IgM Leptospirose kit in sera collected from individuals after recovery from leptospirosis. The solid and broken lines represent the slope and 95% confidence intervals, respectively, obtained by linear regression. Results are expressed as an index ratio of the absorbance (OD450) for the test sample and the kit’s calibrator sample. Index ratios >1.0 were defined as positive reactions.
See this image and copyright information in PMC

References

    1. Abdulkader RC, Daher EF, Camargo ED, Spinosa C, da Silva MV. Leptospirosis severity may be associated with the intensity of humoral immune response. Rev Inst Med Trop Sao Paulo. 2002;44:79–83. - PubMed
    1. Bajani MD, Ashford DA, Bragg SL, Woods CW, Aye T, Spiegel RA, Plikaytis BD, Perkins BA, Phelan M, Levett PN, Weyant RS. Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis. J Clin Microbiol. 2003;41:803–809. - PMC - PubMed
    1. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis. 2003;3:757–771. - PubMed
    1. Cole JR, Jr, Sulzer CR, Pursell AR. Improved microtechnique for the leptospiral microscopic agglutination test. Appl Microbiol. 1973;25:976–980. - PMC - PubMed
    1. Cumberland P, Everard COR, Wheeler JG, Levett PN. Persistence of anti-leptospiral IgM, IgG and agglutinating antibodies in patients presenting with acute febrile illness in Barbados 1979–1989. Eur J Epidemiol. 2001;17:601–608. - PubMed

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