Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Jul 1;63(Pt 7):576-8.
doi: 10.1107/S1744309107026164. Epub 2007 Jun 11.

Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata

Hiroyuki Morita  1 , Shin Kondo, Ryohei Kato, Kiyofumi Wanibuchi, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe, Toshiyuki Kohno
Affiliations

Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata

Hiroyuki Morita et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Polyketide synthase 1 (PKS1) from Huperzia serrata is a plant-specific type III polyketide synthase that shows an unusually versatile catalytic potential, producing various aromatic tetraketides, including chalcones, benzophenones, phlorogulucinols and acridones. Recombinant H. serrata PKS1 expressed in Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 73.3, b = 85.0, c = 137.7 A, alpha = beta = gamma = 90.0 degrees. Diffraction data were collected to 2.0 A resolution using synchrotron radiation at BL24XU of SPring-8.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Proposed mechanism for the formation of (a) naringenin chalcone from 4-coumaroyl-CoA catalyzed by regular CHS and H. serrata PKS1, (b) 4-methoxy-2′,4′,6′-trihydroxychalcone from 4-methoxycinnamoyl-CoA catalyzed by H. serrata PKS1 and (c) 1,3-dihydroxy-N-methylacridone from N-methylanthraniloyl-CoA catalyzed by R. graveolens ACS and H. serrata PKS1.
Figure 2
Figure 2
Crystals of H. serrata PKS1 grown by the hanging-drop method. The dimensions of the crystals were approximately 0.15 × 0.05 × 0.05 mm.
See this image and copyright information in PMC

References

    1. Austin, M. B., Bowman, M. E., Ferrer, J. L., Schröder, J. & Noel, J. P. (2004). Chem. Biol.11, 1179–1194. - PubMed
    1. Austin, M. B., Izumikawa, M., Bowman, M. E., Udwary, D. W., Ferrer, J. L., Moore, B. S. & Noel, J. P. (2004). J. Biol. Chem.279, 45162–45174. - PubMed
    1. Austin, M. B. & Noel, J. P. (2003). Nat. Prod. Rep.20, 79–110. - PubMed
    1. Ferrer, J. L., Jez, J. M., Bowman, M. E., Dixon, R. A. & Noel, J. P. (1999). Nature Struct. Biol.6, 775–784. - PubMed
    1. Jez, J. M., Austin, M. B., Ferrer, J., Bowman, M. E., Schroder, J. & Noel, J. P. (2000). Chem. Biol.7, 919–930. - PubMed

Publication types

MeSH terms