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. 1991 Dec 20;222(4):869-71.
doi: 10.1016/0022-2836(91)90579-u.

Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa

S A Wilson  1 , N E Chayen, A M Hemmings, R E Drew, L H Pearl
Affiliations

Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa

S A Wilson et al. J Mol Biol. .

Abstract

The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P4(2)2(1)2 (a = 104.4 A, c = 66.6 A) with one subunit in the asymmetric unit. Crystals diffract beyond 2.8 A.

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