[Usefulness of CK-MM isoforms for early stage of acute myocardial infarction using electrophoretic technique]

Abstract

MM isoform of CK (EC 2.7.3.2) was able to detect by high voltage electrophoresis. Sequential blood samples were collected from the patients with acute myocardial infarction and MM isoform (as MM3/MM1 ratio), CK activity and CK-MB activity were tested. Time of the maximum MM3/MM1 ratio from the onset was 9.4 hrs (average of 16 cases) whereas 15.9 hrs on CK-MB activity and 17.3 hrs on CK activity were detected. In vitro time course of CK-MM isoform from myocardial and skeletal muscle extracts was tested. MM3 band was gradually converted into MM2 and then MM1 band, but MM3 isoform from myocardium was changed less than skeletal muscle. From these results, it is suggested that abnormal MM3/MM1 ratio on myocardial infarction continues relatively longer time than that on skeletal muscle disease.