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. 2008 Feb;134(2):163-7.
doi: 10.1007/s00432-007-0265-2. Epub 2007 Jul 11.

Expression of Krüppel-like factor 5 in human gastric carcinomas

Mi Kyung Kwak  1 , Hyuk-Joon Lee, Keun Hur, Do Joong Park, Hye Seung Lee, Woo Ho Kim, Kuhn Uk Lee, Kuk Jin Choe, Parry Guilford, Han-Kwang Yang
Affiliations

Expression of Krüppel-like factor 5 in human gastric carcinomas

Mi Kyung Kwak et al. J Cancer Res Clin Oncol. 2008 Feb.

Abstract

Purpose: Krüppel-like factor 5 (KLF5) is a zinc finger transcription factor, which has recently attracted attention because of its important regulatory activities linked to diverse functions such as cell growth, proliferation, differentiation, and tumorigenesis in a number of systems. However, its expression in human gastric cancer has not been described previously. In this study, we investigated the expression profile of KLF5 and the relationship between its clinicopathologic features and expression in gastric carcinomas.

Methods: Tissues were obtained from 247 gastric carcinoma patients who underwent curative gastrectomy (R0 resection) at the Department of Surgery, Seoul National University Hospital from January 1995 to June 1995, and these tissues were arranged in tissue array blocks. KLF5 expression was analyzed by immunohistochemical staining using anti-BTEB2 mouse monoclonal antibodies (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA).

Results: Overall KLF5 was found to be expressed in 45.7% (113/247) of tumor tissues. Moreover, its expression rate was significantly high in early-staged gastric cancer (63.2 vs. 38.0%, p < 0.001), in gastric cancer without lymph node metastasis (54.0 vs. 40.1%, p = 0.04), and in tumors <5 cm in size (53.0 vs. 38.1%, p = 0.02). The 5-year survival rate of patients with KLF5-positive tumors was higher than those of patients with KLF5-negative tumors, although this was not statistically significant (74.7 vs. 62.2%, p = 0.057).

Conclusion: KLF5 expression rate was high in early-staged gastric cancer, in small gastric cancer tissues and in gastric cancer without lymph node metastasis. By univariate analysis, its expression was found to favor survival after surgery. Our study describes for the first time the expression profile of KLF5 in a large number of human gastric cancer tissues and suggests consistent results shown in many recent studies that reduction of KLF5 expression occurs in many types of human tumor.

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Figures

Fig. 1
Fig. 1
Immunohistochemical staining for KLF5 in human gastric tissues. a KLF5 positive staining was observed in normal gastric tissue. b, c KLF5 negative staining and KLF5 positive staining were observed in gastric cancer tissues, respectively
Fig. 2
Fig. 2
Patient survival analysis according to the expression of KLF5 in gastric cancer tissues. Prognosis of patients with a KLF5-positive tumor was better than those with a KLF5-negative tumor
See this image and copyright information in PMC

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