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. 2006:40:387-91.
doi: 10.1007/978-1-4020-4896-8_31.

Colony survival assay

Laura J Simpson  1 , Julian E Sale
Affiliations

Colony survival assay

Laura J Simpson et al. Subcell Biochem. 2006.

Abstract

For studies of DNA repair networks the sensitivity of mutants and combinations of mutants to varying forms of DNA damaging agents has formed a mainstay of genetic analysis in bacteria and yeast. Likewise, this form of epistasis analysis has proved immensely informative in DT40. Because DT40 is non-adherent, it is necessary to restrict the movement of cells by growing them in a viscous medium containing methylcellulose. Here we present methods for carrying out DNA damage survival assays in DT40 with chemical mutagens, ionising radiation and ultraviolet irradiation.

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