Nonomuraea bangladeshensis sp. nov. and Nonomuraea coxensis sp. nov

Abstract

Two novel bacterial strains were isolated from sandy soil from Cox's Bazar, Bangladesh. Strains 5-10-10(T) and 5-38-42(T) were Gram-positive, aerobic, non-motile actinomycetes that form branched substrate and aerial mycelium. On the basis of 16S rRNA gene sequence similarity studies, the novel strains were shown to belong to the genus Nonomuraea, being most closely related to Nonomuraea fastidiosa. Chemotaxonomic data supported the assignment of the novel strains as members of the genus Nonomuraea. Strain 5-10-10(T) contained MK-9(H(4)) and strain 5-38-42(T) contained MK-9(H(6)) and MK-9(H(4)) as the major menaquinones. Major polar lipids were phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The major cellular fatty acid for strain 5-10-10(T) was iso-C(16 : 0) (26.4 %); C(16 : 0) (17.4 %) was the major cellular fatty acid for strain 5-38-42(T). The results of DNA-DNA hybridization and physiological tests enabled strains 5-10-10(T) and 5-38-42(T) to be differentiated genotypically and phenotypically from each other and from the closely related species, N. fastidiosa. On the basis of these results, strains 5-10-10(T) and 5-38-42(T) represent two novel species of the genus Nonomuraea. Following an evaluation of morphological, physiological and chemotaxonomic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization experiments, the new isolates are proposed as two novel species, Nonomuraea bangladeshensis sp. nov. [type strain, 5-10-10(T) (=MTCC 8089(T)=JCM 13930(T))] and Nonomuraea coxensis sp. nov. [type strain, 5-38-42(T) (=MTCC 8090(T)=JCM 13931(T))].