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. 2007 Nov;55(11):1089-94.
doi: 10.1369/jhc.7A7188.2007. Epub 2007 Jul 11.

Septin expression in proliferative retinal membranes

Xiaorong Xin  1 , Mona Pache, Barbara Zieger, Ingrid Bartsch, Christian Prünte, Josef Flammer, Peter Meyer
Affiliations

Septin expression in proliferative retinal membranes

Xiaorong Xin et al. J Histochem Cytochem. 2007 Nov.

Abstract

We undertook this study to evaluate the expression of septin family members SEPT5, SEPT8, and SEPT11 in proliferative retinal membranes. Epiretinal membranes (ERM) were obtained from seven patients with proliferative vitreoretinopathy (PVR) and from four patients and four postmortem eyes with proliferative diabetic retinopathy (PDR). Subretinal membranes (SRM) were obtained from one patient and six postmortem eyes. Membranes were examined by immunohistochemical staining of paraffin sections using polyclonal antibodies against SEPT5, SEPT8, and SEPT11 and an ABC detection system. SEPT8 expression was detected in all ERM and SRM, with an exceptionally strong expression of 100% for ERM of PVR, 63% for PDR membranes, and 57% for SRM. SEPT11 was identified in 91% of all cases, with strong expression of 14%, 25%, and 14% in ERM of PVR, PDR, and SRM, respectively. SEPT5 was seen in 54% of all cases; strong immunostaining was found in only one case of PVR membranes. Our finding suggests a role for members of the septin family in the development of proliferative retinal membranes.

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Figures

Figure 1
Figure 1
Expression of SEPT5 in proliferative retinal membranes. PDR, proliferative diabetic retinopathy; PVR, proliferative vitreoretinopathy; SRM, subretinal membrane. +++, strong expression; ++, moderate expression; +, weak expression; −, absent expression.
Figure 2
Figure 2
SEPT5 expression in an epiretinal membrane (ERM) of PVR.
Figure 3
Figure 3
Expression of SEPT8 in proliferative retinal membranes. PDR, proliferative diabetic retinopathy; PVR, proliferative vitreoretinopathy; SRM, subretinal membrane. +++, strong expression; ++, moderate expression; +, weak expression; −, absent expression.
Figure 4
Figure 4
SEPT8: mild (+, A), moderate (++, B), and strong (+++, C) and a negative control (−, D) in a SRM (A, B) and PDR (C).
Figure 5
Figure 5
Expression of SEPT11 in proliferative retinal membranes. PDR, proliferative diabetic retinopathy; PVR, proliferative vitreoretinopathy; SRM, subretinal membrane. +++, strong expression; ++, moderate expression; +, weak expression; −, absent expression.
Figure 6
Figure 6
SEPT11 expression in an ERM of PDR.
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