Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Jul 12:5:23.
doi: 10.1186/1476-7120-5-23.

Noninvasive monitoring of myocardial function after surgical and cytostatic therapy in a peritoneal metastasis rat model: assessment with tissue Doppler and non-Doppler 2D strain echocardiography

Jens Hartmann  1 , Fabian Knebel, Stephan Eddicks, Mark Beling, Andrea Grohmann, Alexander Panda, Christoph A Jacobi, Joachim M Müller, Klaus-Dieter Wernecke, Gert Baumann, Adrian C Borges
Affiliations

Noninvasive monitoring of myocardial function after surgical and cytostatic therapy in a peritoneal metastasis rat model: assessment with tissue Doppler and non-Doppler 2D strain echocardiography

Jens Hartmann et al. Cardiovasc Ultrasound. .

Abstract

Objective: We sought to evaluate the impact of different antineoplastic treatment methods on systolic and diastolic myocardial function, and the feasibility estimation of regional deformation parameters with non-Doppler 2D echocardiography in rats.

Background: The optimal method for quantitative assessment of global and regional ventricular function in rats and the impact of complex oncological multimodal therapy on left- and right-ventricular function in rats remains unclear.

Methods: 90 rats after subperitoneal implantation of syngenetic colonic carcinoma cells underwent different onclogical treatment methods and were diveded into one control group and five treatment groups (with 15 rats in each group): group 1 = control group (without operation and without medication), group 2 = operation group without additional therapy, group 3 = combination of operation and photodynamic therapy, group 4 = operation in combination with hyperthermic intraoperative peritoneal chemotherapy with mitomycine, and group 5 = operation in combination with hyperthermic intraoperative peritoneal chemotherapy with gemcitabine, group 6 = operation in combination with taurolidin i.p. instillation. Echocardiographic examination with estimation of wall thickness, diameters, left ventricular fractional shortening, ejection fraction, early and late diastolic transmitral and myocardial velocities, radial and circumferential strain were performed 3-4 days after therapy.

Results: There was an increase of LVEDD and LVESD in all groups after the follow-up period (P = 0.0037). Other LV dimensions, FS and EF as well as diastolic mitral filling parameters measured by echocardiography were not significantly affected by the different treatments. Values for right ventricular dimensions and function remained unchanged, whereas circumferential 2D strain of the inferior wall was slightly, but significantly reduced under the treatment (-18.1 +/- 2.5 before and -16.2 +/- 2.9 % after treatment; P = 0.001) without differences between the single treatment groups.

Conclusion: It is feasible to assess dimensions, global function, and regional contractility with echocardiography in rats under different oncological therapy. The deformation was decreased under overall treatment without influence by one specific therapy. Therefore, deformation assessment with non-Doppler 2D strain echocardiography is more sensitive than conventional echocardiography for assessing myocardial dysfunction in rats under oncological treatment.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Pw-Doppler measurement of the transmitral flow velocity obtained from the apical view.
Figure 2
Figure 2
Tissue Doppler measurement from the apical view with calculation of systolic and diastolic velocities of the basal segments (septum and lateral wall).
Figure 3
Figure 3
M-Mode from the short axis view with example of calculation of the LV dimensions at baseline.
Figure 4
Figure 4
Boxplot analysis of fractional shortening (FS) in the control and therapy group. The white boxes: before; grey boxes: after therapy.
Figure 5
Figure 5
Boxplot analysis of tricuspid annular plane systolic excursion (TAPSE) in the control and therapy group. The white boxes: before; grey boxes: after therapy.
Figure 6
Figure 6
Boxplot analysis E/E' in the control and therapy group. The white boxes: before; grey boxes: after therapy.
Figure 7
Figure 7
Boxplot analysis of E/A in the control and therapy group. The white boxes: before; grey boxes: after therapy.
Figure 8
Figure 8
Non-Doppler 2D strain measurements of the radial deformation of the anterior and inferior wall at baseline.
Figure 9
Figure 9
A and B Non-Doppler 2D strain measurements of the circumferential deformation of the anterior and inferior wall at baseline (A) and after treatment with reduction of the maximal strain und postsystolic shortening (arrow) in the inferior wall.
Figure 10
Figure 10
Boxplot analysis of non-Doppler radial 2-D strain in the control and therapy group. The white boxes: before; grey boxes: after therapy. The plain boxes represent the anterior, the striped boxes the inferior 2-D strain
Figure 11
Figure 11
Boxplot analysis of non-Doppler circumferential 2-D strain in the control and therapy group. The white boxes: before; grey boxes: after therapy. The plain boxes represent the anterior, the striped boxes the inferior 2-D strain.
See this image and copyright information in PMC

References

    1. Watson LE, Sheth M, Denyer RF, Dostal DE. Baseline echocardiographic values for adult male rats. J Am Soc Echocardiogr. 2004;17:161–167. doi: 10.1016/j.echo.2003.10.010. - DOI - PubMed
    1. Vogel M, Schmidt MR, Kristiansen SB, Cheung M, White PA, Sorensen K, Redington AN. Validation of myocardial acceleration during isovolumic contraction as a novel noninvasive index of right ventricular contractility. Comparison with ventricular pressure-volume relations in an animal model. Circulation. 2002;105:1693–1699. doi: 10.1161/01.CIR.0000013773.67850.BA. - DOI - PubMed
    1. Borges AC, Knebel F, Eddicks S, Panda A, Schattke S, Witt C, Baumann G. Right ventricular function assessed by two-dimensional strain and tissue Doppler echocardiography in patients with pulmonary arterial hypertension and effect of vasodilator therapy. Am J Cardiol. 2006;98:530–534. doi: 10.1016/j.amjcard.2006.02.060. - DOI - PubMed
    1. Leitman M, Lysyansky P, Sidenko S, Shir W, Peleg E, Binenbaum M, Kaluski E, Karkover R, Vered Z. Two-dimensional strain a novel software for real-time quantitative echocardiographic assessment of myocardial function. J Am Soc Echocardiogr. 2004;17:1021–1029. doi: 10.1016/j.echo.2004.06.019. - DOI - PubMed
    1. Heimdahl A, Stoylen A, Torp H, Skjaerpe T. Real-time strain rate imaging of the left ventricle by ulrasound. J Am Soc Echocardiogr. 1998;11:1013–1019. doi: 10.1016/S0894-7317(98)70151-8. - DOI - PubMed

MeSH terms

Substances