[Effect of antioxidants on the oxidative stress of red blood cells]

Abstract

Antioxidant nomination covers a substance group of great variety, therefore, the determination of action mechanism of new antioxidants requires a complicated test system. The present work demonstrates this labour and time consuming procedure conducted on human red blood cells (RBCs) exposing them to "stress effect" with diluted hydrogen peroxide. It was studied how did these oxidative stress conditions affect the filtration parameters, lipid peroxidation, reduced glutathione content and superoxide dismutase activity of RBCs. It was attempted to reverse or influence the significant changes caused by "oxidative stress" with known antioxidants (vitamin E and uric acid).