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. 1991 Dec 25;19(24):6819-21.
doi: 10.1093/nar/19.24.6819.

Requirement of protein factors and ATP for the disassembly of the spliceosome after mRNA splicing reaction

H Sawa  1 , Y Shimura
Affiliations
Free PMC article

Requirement of protein factors and ATP for the disassembly of the spliceosome after mRNA splicing reaction

H Sawa et al. Nucleic Acids Res. .
Free PMC article

Abstract

Pre-mRNA splicing reaction occurs in a large ribonucleoprotein complex called the spliceosome. After the splicing reaction, the spliceosome is disassembled to release the splicing products including spliced mRNA. Here we show that protein factors in a HeLa nuclear extract or a DEAE-cellulose fraction as well as ATP are required to release the splicing products form the spliceosome in which the splicing reaction has already completed.

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References

    1. Cell. 1991 May 3;65(3):483-92 - PubMed
    1. Nature. 1991 Feb 7;349(6309):487-93 - PubMed
    1. Nucleic Acids Res. 1991 Jul 25;19(14):3953-8 - PubMed
    1. Cell. 1985 Oct;42(3):725-36 - PubMed
    1. Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 - PubMed

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